Products & Technical Data

Comparative Activity of CYP1A2

Comparative Kinetic Analysis of CYPDESIGN's human CYP1A2 Sacchrosomes™ (from yeast) versus other supplier's CYP1A2 microsomes (from insect cells).

CEC as substrate; flourescent product

CYP1A2 Graph

Comparative Activity of CYP2D6

Comparative Kinetic Analysis of CYPDESIGN's human CYP2D6 Sacchrosomes (from yeast) versus other supplier's CYP2D6 microsomes (from insect cells).

EOMCC as substrate; flourescent product

CYP2D6 Graph fig: 1

Comparative Activity of CYP3A4

Comparative Kinetic Analysis of CYPDESIGN's human CYP3A4 Sacchrosomes™ (from yeast) versus other supplier's CYP3A4 microsomes (from insect cells).

DBOMF as substrate; flourescent product (fluoroscein)

CYP3A4 Graph

Comparative Activity of CYP1A1

Comparative Kinetic Analysis of CYPDESIGN's human CYP1A1 SacchrosomesTM (from yeast) versus other supplier's CYP1A1 microsomes (from insect cells).

7-ethoxyresorufin as substrate; fluorescent product.

CYP1A1 Graph

Comparative Activity of CYP1B1

Comparative Kinetic Analysis of CYPDESIGN's human CYP1B1 SacchrosomesTM (from yeast) versus other supplier's CYP1B1 microsomes (from insect cells).

7-ethoxyresorufin as substrate; fluorescent product.

CYP1B1 Graph

Comparative Activity of CYP2C8

Comparative Kinetic Analysis of CYPDESIGN's human CYP2C8 SacchrosomesTM (from yeast) versus other supplier's CYP2C8 microsomes (from insect cells).

Dibenzylfluoroscein as substrate; fluorescent product.

CYP2C8 Graph

Comparative Activity of CYP2C9

Comparative Kinetic Analysis of CYPDESIGN's human CYP2C9 SacchrosomesTM (from yeast) versus other supplier's CYP2C9 microsomes (from insect cells).

CEC as substrate; fluorescent product.

CYP2C9 Graph

Comparative Activity of CYP2E1

Comparative Kinetic Analysis of CYPDESIGN's human CYP2E1 SacchrosomesTM (from yeast) versus other supplier's CYP2E1 microsomes (from insect cells).

EOMCC as substrate; fluorescent product.

CYP2E1 Graph

Comparative Activity of CYP2A6

Comparative Kinetic Analysis of CYPDESIGN's human CYP2A6 SacchrosomesTM (from yeast) versus other supplier's CYP2A6 microsomes (from insect cells).

Coumarin as substrate; fluorescent product.

CYP2A6 Graph

CYPs & Formats

All major human drug metabolising CYPs are available, with exact amounts of each CYP aliquotted into wells of a microtitre plate. CYPs are provided as dried down films, ready for reconstitution and immediate use. There's no need to pipette viscous, heterogeneous solutions.

Arrayed into the format of your choice, whether vials, 96- or 384-well microtitre plates, there's no need for low temperature shipping or storage. Our products are ready to use off the shelf.

All CYP products contain NADPH-dependent P450 reductase, and cytochrome b5 where necessary, and are presented as lyophilised films ready for immediate reconstitution and use in your assays.

Comparative Performance

Yeast microsomes are more similar to the mammalian system than those from insect cells.

CYPDESIGN has demonstrated that the yield and activity of our human CYP enzymes in Sacchrosomes™ (microsomes) are at least comparable and often better than those from other suppliers who use insect cells or bacteria.

Representative kinetic plots for each of nine different human CYPs are shown under CYPs & Formats, comparing the activities from CYPDESIGN's Sacchrosomes™ with those from a leading supplier's insect cell-expressed CYPs. More detailed comparisons are available in product data sheets accompanying each individual Sacchrosome™ CYP.  Please use the Contact Form to make a request.

Stabilisation Process

CYPDESIGN has developed a unique stabilisation process that allows CYPs, which are ordinarily extremely labile at temperatures other than -80ºC, to be maintained at room temperature, greatly increasing their convenience for the end-user. They can thus be shipped and stored without the need for a cold chain.

Protocols

Protocols are available for all of our CYP products. These will be made available on this web site, but please contact us for information in the mean time.

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